Part:BBa_K4904004

pbridge-BD

Basic Part: BBa_K4904004 (pbridge-BD)

Profile

Name: pbridge-BD

Base Pairs: 6526 bp

Origin: Plants, Synthetic

Properties

Blue light receptor mediating blue-light regulated cotyledon expansion and flowering time.

Usage and Biology

Blue light receptor mediating blue-light regulated cotyledon expansion and flowering time. Positive regulator of the flowering-time gene CONSTANS. This gene possesses a light-induced CNT2 N-terminal homodimerisation domain. Involved in blue-light-induced stomatal opening. Involved in triggering chromatin decondensation. An 80-residue motif (NC80) is sufficient to confer CRY2's physiological function. It is proposed that the PHR domain and the C-terminal tail of the unphosphorylated CRY2 form a ''closed'' conformation to suppress the NC80 motif in the absence of light. In response to blue light, the C-terminal tail of CRY2 is phosphorylated and electrostatically repelled from the surface of the PHR domain to form an ''open'' conformation, resulting in derepression of the NC80 motif and signal transduction to trigger photomorphogenic responses. Cry2 phosphorylation and degradation both occur in the nucleus.[1]

Figure 1. Blue light receptor pbridge-BD

References:

1. Xu W, Bochen J, Lianfeng G, et al. A photoregulatory mechanism of the circadian clock in Arabidopsis[J]. Nature Plants, 2021, 7(10).

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal NotI site found at 2700
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BglII site found at 2718
  Illegal BamHI site found at 888
  Illegal XhoI site found at 651
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal NgoMIV site found at 1112
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 570
  Illegal BsaI.rc site found at 4255
  Illegal SapI site found at 3172